Translational_Unit

Part:BBa_K1465202:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-09-02)


Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 809
    Illegal BamHI site found at 1490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_ccbLS pSB1C3_pre_ccbLS] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_ccbLS pSB1C3_suf_ccbLS]:
pSB1C3_pre_ccbLS (44 bp)

5'-CATCTAGCGGCCGCTACTAGTATTAGATCTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_ccbLS (43 bp)

5'-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the gBlock containing the optimized coding sequence of the Ribulose-1,5-bisphosphate carboxylase/oxygenase (ccbLS) from Halothiobacillus neapolitanus.

Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the codon optimized sequence for Escherchia coli by using the [http://www.jcat.de/ Codon Adaptation Tool] and the [http://eu.idtdna.com/CodonOpt Codon Optimization from IDT]:


BILD

References